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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which results in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two supplemental splice variants were being claimed but specific nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope recognized with the antibody.
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This guidebook outlines additional doable optimization of cellular and immunoassay parameters to guarantee the best possible effects are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is distinct.
Target Data Cyclin E more info belongs to the hugely conserved cyclin family, whose customers are characterised by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show distinct expression and degradation designs which lead into the temporal coordination of each mitotic function. Cyclin E varieties a posh with and functions as being a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates on the G1-S stage boundary which is degraded as cells development as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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